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Спутники
РН
SpaceX
Роскосмос
NASA
СпутникиРНSpaceXРоскосмосNASARapid, sensitive, and accessible pathogen diagnostics are urgently needed to address the global health burden of infectious diseases and the growing threat of antimicrobial resistance. Conventional methods, such as culture-based assays and PCR, remain limited by long turnaround times, sophisticated instrumentation, and restricted multiplexing capabilities, which make them impractical for deployment in resource-limited or point-of-care (POC) settings.
This dissertation presents the development of integrated microfluidic platforms coupled with biochemical assays based on recombinase polymerase amplification (RPA) and CRISPR/Cas12a detection for rapid, portable, and instrument-free diagnostics. A series of microdevices were engineered to overcome limitations in fluid transport, sample processing, and assay integration. First, a Rotation-Chip was designed as a low-cost, reusable platform that enables high-throughput multiplexing through manual fluid transfer. Leveraging superhydrophobic...