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Dynamics of solid-state fermentation of Agave durangensis bagasse: nutritive quality and in vitro methane emissions

Дата публикации: 20-08-2026 00:00:00

Agave durangensis bagasse is characterized by high fiber content and low digestibility. Solid-state fermentation (SSF) improves its nutritional value. The aim of this study was to evaluate the effect of Trichoderma sp. on the SSF dynamics of Agave durangensis bagasse. Thirty grams of sterile bagasse were fermented in 250 mL flasks and inoculated with 5 mL of a spore suspension (1 × 10⁷ spores.mL⁻¹). Eight fermentation times (0, 24, 48, 72, 96, 120, 144, and 168 h), plus an unfermented control, were evaluated with three replicates per treatment. Proximate chemical composition, cell wall components, in vitro dry matter digestibility (IVDMD), and gas, methane (CH₄), and carbon dioxide (CO₂) production were determined. Gas production data were fitted to the Gompertz model. SSF with Trichoderma sp. increased crude protein, ash content, and IVDMD (8.8, 9.4, and 73.7 %, respectively), as well as the gas production rate (k = 0.064 h-1). In addition, it reduced neutral detergent fiber, acid detergent fiber, and lignin contents (52.2, 40.6, and 8.1 %, respectively), together with CH₄ and CO₂ production (1.3 and 16.4 mL.g⁻¹ DM, respectively). SSF of Agave durangensis bagasse with Trichoderma sp. improves its nutritional value while reducing in vitro CH₄ and CO₂ emissions, representing a promising strategy for the valorization of this agro-industrial residue as cattle feed.

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Keywords: chemical composition, digestibility, cell wall components, gas production, Trichoderma sp

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